QuickZyme Mouse MMP-9 Activity Assay Kit, 96-Assays. Enables measurement of both active mouse MMP-9, as well as (pro)MMP-9 (which is activated on the plate by APMA) in a broad range of sample types (ex. conditioned culture media, tissue homogenates, serum, plasma and urine). This 96-well plate format assay uses a modified pro-enzyme as a substrate, which upon activation is able to release color from a chromogenic peptide substrate. This multiplication step provides a unique assay sensitivity. Calibration range: 0-4 ng/ml. Detection limit: 20 pg/ml (1 hr incubation); 1 pg/ml (6 hr incubation). Sample volume: 10-100 ul. All standards, buffers and plates are included: Pre-coated 96-well microplate, assay buffer, APMA, pro-detection enzyme, peptide substrate, wash buffer, mouse MMP-9 standard. Easy, sensitive, and quantitative replacement for zymography. Synonyms: Matrix metalloproteinase-9, MMP9, 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, GELB.